Quail imaging offers insights into congenital birth defects

Researchers at The University of Queensland have for the first time captured images and video in real time of early embryonic development to understand more about congenital birth defects.

Dr Melanie White and Dr Yanina Alvarez from UQ’s Institute for Molecular Bioscience used quail eggs to understand how cells begin to form tissues such as the heart, brain and spinal cord.

Dr White said congenital birth defects affect 3 per cent of Australian babies with heart defects the most common and neural tube defects second.

“Because quails grow in an egg, they’re very accessible for imaging and their early development is very similar to a human at the time the embryo implants in the uterus,” Dr White said.

“For the first time we have seen high-resolution, real-time imaging of important early developmental processes.

“Until now, most of our knowledge of post-implantation development came from studies on static slides, at fixed points in time.”

The IMB researchers have generated quails with a fluorescent protein to reveal the structure, called the actin cytoskeleton, which gives cells shape and facilitates movement.

“When cells migrate during early development, they stick out protrusions called lamellipodia and filopodia like arms that reach out and grab onto surfaces allowing the cells to crawl, or reach other cells to bring them closer together,” Dr White said.

“We were able to image the filopodia from heart stem cells deep inside the embryo as they first made contact by sticking out protrusions and gripping to their surroundings and each other to form the early heart.

“It’s the first time anyone has captured the cell’s actin cytoskeleton facilitating this contact in live imaging.”

The researchers also imaged the open edges of the neural tube and it being ‘zipped up’ to begin to form the brain and spinal cord.