Rice University Launches Synthetic Biology Institute with a Mission to Enhance Lives through Innovation

Rice University has launched the Rice Synthetic Biology Institute, which aims to catalyze collaborative research in synthetic biology and its translation into technologies that benefit society.

“The Rice Synthetic Biology Institute demonstrates how everyone benefits when the top minds in natural sciences and engineering collaborate at a leading research university,” said President Reginald DesRoches. “This research institute will draw global attention to the great work that is happening in our highly regarded synthetic biology program at Rice and it will help us continue to attract and retain the best talent in the field.”

Synthetic biology is an interdisciplinary discipline where researchers design living systems with new properties to address societal needs. With more than 18 faculty and over 100 students and postdoctoral scholars across the Schools of Engineering and Natural Sciences connected to this expanding field, RSBI plans to strengthen this community at Rice, connect basic and translational research, and elevate Rice’s international visibility in this space. This follows a 2018 university investment in strategic initiatives aimed at propelling faculty and programs supporting synthetic biology.

The institute is led by Caroline Ajo-Franklin, professor of biosciences, bioengineering, and chemical and biomolecular engineering, with the support of an interdisciplinary faculty steering committee.

“At Rice, we have such deep expertise in synthetic biology,” said Ajo-Franklin. “Connecting that deep expertise through this institute will lead to better science and more innovation.”

“Rice University is an amazing place to learn, teach, research and innovate,” said Ramamoorthy Ramesh, executive vice president for research. “The Rice Synthetic Biology Institute will ensure that our researchers are recognized on the international stage for the life-changing work they are doing in Houston and around the world.”

The institute will support research to understand how to design new types of living systems for applications in medicine, manufacturing and environmental sustainability. Four research themes will be the initial focus of RSBI, leveraging Rice’s unique strengths:

• Controlling the biological synthesis and patterning of proteins and cells into living materials that self-replicate and self-repair across a range of length scales
• Understanding cells as natural sensors and repurposing them into living therapeutics to detect and treat diseases, maintain health and prevent infections
• Developing living electronics to convert biochemical information into information-dense electronic signals in real time at the cell-material interface
• Supporting cross-cutting scholarship aimed at accelerating the Design-Build-Test-Learn cycle and understanding the ethical, legal and social implications of translating these technologies into the public domain.

Rice Scientists Harness Blood Test to Monitor Gene Expression in the Brain

The brain is the most protected organ in the body, sheathed in a complex and nearly impenetrable barrier of specialized blood vessels. While this particular anatomical setup protects it from outside invaders, it also makes it difficult for researchers to study how specific genes are expressed ⎯ and how such changes in gene expression may lead to disease.Now Rice University scientists have developed a noninvasive way to monitor gene expression dynamics in the brain, making it easier to investigate brain development, cognitive function and neurological diseases, according to a study published in Nature Biotechnology.

Rice bioengineer Jerzy Szablowski and colleagues have engineered a unique class of molecules, known as released markers of activity (RMAs), that can be used to measure gene expression in the brain through a simple blood test.

“Typically, if you wanted to look at gene expression in the brain, you would have to wait to do a post-mortem analysis,” said Szablowski, an assistant professor of bioengineering at Rice’s George R. Brown School of Engineering. “There are some more modern neuroimaging techniques that can do this, but they lack sensitivity and specificity to track changes in specific cell types.

“With the RMA platform, we can introduce a synthetic gene expression reporter to the brain, which produces a protein that can pass through the blood-brain barrier. We can then measure changes in expression for a gene of interest with a simple blood test.”

Szablowski first considered the possibility of a synthetic gene expression reporter after noting the brain would quickly clear antibody therapy injections.

“Whenever these injections were done, the antibodies would just disappear ⎯ they wouldn’t hang around long enough in the brain for an effective therapy,” he explained. “But we thought the failure of antibody therapies could be repurposed to our advantage. What if we took the part of the antibody responsible for this escape and attached it to a protein that could be easily detected? We could then see where, when and how much of a particular gene was being expressed in the brain.”

Other researchers had already determined that antibodies make their way through the blood-brain barrier using the neonatal fragment crystallizable receptor (FcRn), a gene known for helping maintain the level of antibodies present across the body. Using sophisticated bioengineering techniques, Szablowski and team attached the part of the antibody that helps it get through the blood-brain barrier to a common reporter protein to take advantage of this biological escape hatch. When the group then connected the RMAs to a specific gene and expressed that genein the brain of a mouse, they were able to see that expression mirrored in the animal’s blood.

“This method is very sensitive and can track changes in specific cells,” Szablowski said. “Producing this protein in approximately 1% of the brain raised its blood levels up to 100,000-fold compared to baseline. We could specifically track the expression of this one protein with just a blood test.”

For now, Szablowski sees RMAs as a vital research tool to help scientists better monitor gene expression in the brain. For example, he said, the RMA platform could be used to look at how long novel gene therapies stay the brain over time.“We could track these new therapies with just a blood test and continue to monitor them over time since the RMA platform is noninvasive,” he said. “But we can also use RMAs to study gene expression as it relates to disease. Being able to track different gene expression changes will allow us to understand what leads to disease and how the disease itself changes gene expression in the brain. This could provide new clues for drug development, or even for how to prevent neurological diseases in the first place.”