University of Copenhagen: New revelations about an ancient fish- the secret to swimming upside down

NATURE An after-hours trip to Aarhus University Hospital Skejby’s radiology department has shed light on a mysterious and ancient fish, one that remains one of the world’s rarest – the Coelacanth. Researchers from the University of Copenhagen and Aarhus University have removed Denmark’s only specimen of this primordial fish from its jar of alcohol and gained new insight into how it functions. The new knowledge could contribute to saving this critically endangered deep sea dweller.

When a South African fisherman came across a coelacanth in his net in 1938, it was like finding a living dinosaur – a catch that sent shockwaves throughout the scientific community. Until then, the coelacanth (SEE-lə-kanth) was believed to have been extinct for 66 million years. Since then, only about 300 specimens of this living fossil have been caught worldwide. In Denmark, a single fish, “specimen number 23”, has been submersed in alcohol at the Zoological Museum in Copenhagen for 60 years.

As many other coelacanth specimens have been dissected, its anatomy is no secret. But very little is known about the fish’s physiology – the way it functions. Now, researchers from the University of Copenhagen and Aarhus University have discovered new things about this extraordinarily rare and elusive deep-sea dweller.

“This fish is iconic, extremely rare and remains shrouded in mystery. It is difficult to observe alive because it lives in underwater caves at depths of 150-200 meters. And, since a large portion of the few specimens collected over the years have been cut to pieces, new methods were required to find out more about how it lives. We now know a bit more,” says Peter Rask Møller, an associate professor and curator at the University of Copenhagen’s Natural History Museum of Denmark. He is a co-author of the research article just published in the journal BMC Biology.

Along with Henrik Lauridsen of Aarhus University and a group of colleagues, Møller is the first person ever to conduct research on the Danish specimen, which has been preserved intact and only been used for display until now.

STORY OF THE DANISH COELACANTH
The Danish coelacanth specimen – specimen no. 23 – was caught in the Indian Ocean’s Comoros archipelago in 1960. At the time, the Comoros was a French colony.
Danish oceanographer Anton Bruun, leader of the Galathea-2 expedition among other things, persuaded France to donate the specimen to the Zoological Museum in 1962.
With the donation came strict requirements that the specimen was only to be used as a display item and could under no circumstances be subjected to dissection.
While this requirement was unofficially lifted in 1975 when the Comoros gained independence from France, Copenhagen chose to honour the original agreement. Consequently, specimen 23 was kept intact.
Over the years, the specimen has been exhibited at the Zoological Museum and the former Denmark’s Aquarium.
The secret to swimming upside down
By putting the fish in CT and MRI scanners at Aarhus University Hospital in Skejby, the research scientists could model the species with more precision than ever, while not destroying the fish. The models show the exact distribution of bone mineral and fat in its body. Among other things, the models help explain the unique “headstand drift hunting” technique of the coelacanth, whereby it slowly drifts along a seabed vertically, head and snout downwards, as it uses an electrosensitive organ to scan the bottom for cephalopods and fish to eat.

“We discovered that the coelacanth has a special skeleton with a lot of bone mass in the head and tail, while there are almost no vertebrae. It’s quite unique. The heaviest parts are at either end of the fish, which makes it easy for the fish to stand itself on its head. The balance point is an advantageous mechanism for its way of life,” explains Associate Professor Henrik Lauridsen from Aarhus University’s Department of Clinical Medicine.

The researchers also discovered the precise distribution of fatty tissue in the body of the fish, including the amount in its fatty bladder, as coelacanth don’t have a regular gas swim bladder like modern fish. The numbers show that the fat content correlates with the depths at which the fish live, where fat allows the fish to be neutrally buoyant and spend hardly any energy to remain hundreds of meters deep.

CT image of bone mineral density of the coelacanth
CT image showing the bone mineral density of the coelacanth (credit: Henrik Lauridsen)
Pregnant for five years and births live young
One peculiar feature about the coelacanth is that females gestate for a full five years before birthing live young. One of the great coelacanth mysteries among researchers is: Where do coelacanth give birth? The Danish researchers hope to shed light on this question soon.

FACTS ABOUT COELACANTH
There are two living species of coelacanth – Latimeria chalumnae, which lives off the coast of East Africa, and Latimeria menadoensis, which lives off Indonesia.
The oldest known coelacanth fossils are more than 410 million years old.
Just over 300 live specimens of coelacanth are believed to have been caught worldwide.
Coelacanth are pregnant for five years and birth live young.
Coelacanth grow up to 2 meters long and weigh up to 100 kilograms.
“We still have no idea where their fry are born. By analyzing the distribution of bone and fat in a fetus, we can probably find out at what depth fry are adapted to live. This knowledge is also important in terms of preserving this critically endangered species – because when we don’t know where they are, we can’t know where to protect them. And, there is cause for concern. Coelacanth have an incredibly slow reproduction rate, which makes them extra vulnerable,” says Henrik Lauridsen.

The researchers point out that their models can be applied to all other aquatic organisms and used to determine, among other things, whether organisms are adapted to the ocean depths at which they live. This is relevant knowledge at a time when climate change could cause ocean currents to change and thereby impact marine life.

“Instead of going out and catching new coelacanth, which are both rare and protected, the modern scanning techniques have allowed us to perform exciting new analyses, despite the animal having been submersed in alcohol for decades. Having museum collections of rare animals is like entering the world’s best secondhand store, with the wildest array of recycled things that can no longer be found new,” concludes Peter Rask Møller.